HOT and BATS Prochlorococcus Abundance Time-series¶
|Dataset Name||Sensor||Make||Spatial Resolution||Temporal Resolution||Start Date||End Date|
|HOT and BATS Prochlorococcus Abundance Time-series||Observation||Irregular||Irregular||2002-11-04||2008-01-31|
Five year time-series containing Prochlorococcus ecotype abundance sampled each month at HOT and BATS sites from 2002-2008 The goal of this study was to collect long-term, high-resolution data on the temporal and spatial variability of Prochlorococcus ecotypes in the Pacific and Atlantic Oceans. The abundance of five Prochlorococcus ecotypes was determined by quantitative PCR at 12 depths every month for 5 years at two locations: BATS location (5 nautical mile radius around 31 40’N, 64 10’W) HOT Station ALOHA (5 nautical mile radius around 22 45’N, 158 00’ W) Sample processing and analyses are detailed in the publication listed below. Briefly, 100ml of seawater was collected onto 25mm dia polycarbonate filters (0.22um pore size), rinsed with Tris-buffered saline, flash frozen in liquid N2, and stored at -80C until extraction. DNA was extracted using a combination of 5min of bead beating and 15min heat lysis at 95C. Extracted DNA was used as template of quantitative PCR reactions using primers specifically designed to target different Prochlorococcus ecotypes. Standard curves used for quantitation of field data were derived from DNA extracted from cultured representatives belonging to each ecotype. Malmstrom, RR, A Coe, GC Kettler, AC Martiny, J Frias-Lopez, ER Zinser, and SW Chisholm. 2010. Temporal dynamics of Prochlorococcus ecotypes in the Atlantic and Pacific oceans. The ISME Journal. 4(10): 1252-1264
Table of Variables¶
How to Acknowledge¶
The data and data description were downloaded from BCO-DMO (https://www.bco-dmo.org/dataset/3381).
This data came from a Center for Microbial Oceanography: Research and Education (C-MORE) project.
This dataset was submitted by the Chisholm Lab at MIT: https://chisholmlab.mit.edu/.
Metadata associated with the Prochlorococcus data at HOT can be downloaded from http://hahana.soest.hawaii.edu/hot/hot-dogs/bextraction.html.
For details on the methods used to collect data on HOT cruises, see http://hahana.soest.hawaii.edu/hot/methods/results.html.
Metadata associated with the Prochlorococcus data at BATS can be downloaded from http://bats.bios.edu/bats-data/.
For details on the methods used to collect data on BATS cruises, see http://bats.bios.edu/about/cruise-information/.